Hotspot

psbB-psbT

LSC · 75482-75856 · 375 bp

Back to Papilionanthe

Pi 0.0111

MarkerSeek score 64.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 4
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	569-600 bp	1.0	69.3
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	568-599 bp	1.0	67.1
psbB-psbT_p3	GCCAGAACCTTGTTCAGAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	582-613 bp	1.0	67.0
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	531-562 bp	1.0	66.5
psbB-psbT_p5	GCCAGAACCTTGTTCAGAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	581-612 bp	1.0	64.8