Hotspot

rbcL-accD

LSC · 57012-57810 · 799 bp

Back to Papilionanthe

Pi 0.00882

MarkerSeek score 64.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 7
pi: 0.00882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.0	58.0	1007-1009 bp	1.0	80.4
rbcL-accD_p2	TGAAGCTAGCAAATGGAGCC	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	1012-1014 bp	1.0	78.8
rbcL-accD_p3	TTTCGACCCAGTGGATAAGC	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.6	58.0	946-948 bp	1.0	77.6
rbcL-accD_p4	GCTGCTTGTGAAGTCTGGAA	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.4	58.0	981-983 bp	1.0	77.5
rbcL-accD_p5	TCGTGAAGCTAGCAAATGGA	CCACTGGTATGTCCAAGAGC	45.0	55.0	57.2	58.0	1015-1017 bp	1.0	74.6