Hotspot

trnF-GAA-ndhJ

LSC · 49449-49876 · 428 bp

Back to Papilionanthe

Pi 0.0169

MarkerSeek score 67.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 7
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCCGTCAGTTTTCTGGATT	50.0	50.0	58.1	57.8	485-494 bp	1.0	81.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AAACCCCGTCAGTTTTCTGG	50.0	50.0	58.1	58.3	488-497 bp	1.0	80.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCTCAAACCCCGTCAGTTTT	50.0	50.0	58.1	58.3	492-501 bp	1.0	80.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGAGTAATCCTCAAACCCCG	50.0	55.0	58.1	57.7	500-509 bp	1.0	80.1
trnF-GAA-ndhJ_p5	GGAAATGGTCGGGATAGCTC	CCCCGTCAGTTTTCTGGATT	55.0	50.0	57.8	57.8	531-540 bp	1.0	80.0