Hotspot

petN-psbM

LSC · 30093-31052 · 960 bp

Back to Papilionanthe

Pi 0.0119

MarkerSeek score 46.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 960
variable_sites: 7
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCCTGTTTTGACTCTGCACC	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	873-1236 bp	1.0	83.7
petN-psbM_p2	TCCTGTTTTGACTCTGCACC	CGTCAATCCCGATTCGATGA	50.0	50.0	58.0	57.9	1509-1878 bp	1.0	83.3
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	CGTTTATTGCTACTGCGCTG	55.0	50.0	58.1	57.9	707-1070 bp	1.0	83.2
petN-psbM_p4	GGGCATCATTCGCATGGATA	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	786-1149 bp	1.0	82.6
petN-psbM_p5	AGTATGGGGAAGGAGTGGAC	CGTTTATTGCTACTGCGCTG	55.0	50.0	58.1	57.9	1071 bp	0.5	58.4