Hotspot

trnR-UCU-atpA

LSC · 10856-11011 · 156 bp

Back to Papilionanthe

Pi 0.0449
MarkerSeek score 63.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
156
variable_sites
7
pi
0.0449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG CGGCAGCTCAAATTAAAGCC 50.0 50.0 58.0 58.1 804-820 bp 1.0 82.5
trnR-UCU-atpA_p2 AGCCTTCCAAGCTAACGATG CCCTGGTCGTGAAGCTTATC 50.0 55.0 58.0 58.1 1067-1083 bp 1.0 81.9
trnR-UCU-atpA_p3 AGCCTTCCAAGCTAACGATG CCTGGTCGTGAAGCTTATCC 50.0 55.0 58.0 58.1 1066-1082 bp 1.0 81.9
trnR-UCU-atpA_p4 AGCCTTCCAAGCTAACGATG GGTCGTGAAGCTTATCCAGG 50.0 55.0 58.0 58.1 1063-1079 bp 1.0 81.9
trnR-UCU-atpA_p5 CCTAGCCTTCCAAGCTAACG GGTCGTGAAGCTTATCCAGG 55.0 55.0 58.1 58.1 1066-1082 bp 1.0 81.7