Hotspot

trnS-GGA-trnG-UCC

LSC · 8554-9896 · 1343 bp

Back to Papilionanthe

Pi 0.0268
MarkerSeek score 69.70
Primer available yes
Label trnS-GGA-trnG-UCC

Feature Metadata

label_name
trnS-GGA-trnG-UCC
feature_type
igs
region
LSC
length_bp
1343
variable_sites
33
pi
0.0268
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1354-1412 bp 1.0 57.6
trnS-GGA-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1353-1411 bp 1.0 57.6
trnS-GGA-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1357-1415 bp 1.0 56.5
trnS-GGA-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1356-1414 bp 1.0 56.5
trnS-GGA-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1355-1413 bp 1.0 56.5