Hotspot

psbI-trnS-GGA

LSC · 8315-8465 · 151 bp

Back to Papilionanthe

Pi 0.04
MarkerSeek score 68.70
Primer available yes
Label psbI-trnS-GGA

Feature Metadata

label_name
psbI-trnS-GGA
feature_type
igs
region
LSC
length_bp
151
variable_sites
6
pi
0.04
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GGA_p1 ATCTAATGATCCGGGGCGTA TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 264-287 bp 1.0 81.5
psbI-trnS-GGA_p2 TATCTAATGATCCGGGGCGT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 265-288 bp 1.0 81.5
psbI-trnS-GGA_p3 ATCTAATGATCCGGGGCGTA GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 265-288 bp 1.0 81.4
psbI-trnS-GGA_p4 TATCTAATGATCCGGGGCGT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 266-289 bp 1.0 81.4
psbI-trnS-GGA_p5 ATCTAATGATCCGGGGCGTA GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.1 58.2 214-237 bp 1.0 80.2