Hotspot

rpl16

LSC · 83244-84896 · 1653 bp

Back to Paphiopedilum

Pi 0.0223
MarkerSeek score 71.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1653
variable_sites
269
pi
0.0223
unique_haplotype_count
58
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CCAGAGATAAAGAAATCCGCG AATTTAGGTTTCGTATAATTGCT 47.6 26.1 56.9 52.5 1564-2167 bp 0.955224 50.2
rpl16_p2 CCAGAGATAAAGAAATCCGCG CGATTTACTCTTTAATTTAGGTTTCGT 47.6 29.6 56.9 56.8 1577-2180 bp 0.895522 47.8
rpl16_p3 TCCAGAGATAAAGAAATCCGCG CGATTTACTCTTTAATTTAGGTTTCGT 45.5 29.6 58.3 56.8 1578-2181 bp 0.895522 47.8
rpl16_p4 CCAGAGATAAAGAAATCCGCG TCGATTTACTCTTTAATTTAGGTTTCG 47.6 29.6 56.9 56.5 1578-2181 bp 0.895522 47.8
rpl16_p5 TCCAGAGATAAAGAAATCCGCG TCGATTTACTCTTTAATTTAGGTTTCG 45.5 29.6 58.3 56.5 1579-2182 bp 0.895522 47.8