Hotspot

psaC-rps15

IRa · 124497-125146 · 650 bp

Back to Paphiopedilum

Pi 0.0175
MarkerSeek score 76.10
Primer available yes
Label psaC-rps15

Feature Metadata

label_name
psaC-rps15
feature_type
igs
region
IRa
length_bp
650
variable_sites
99
pi
0.0175
unique_haplotype_count
57
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-rps15_p1 AATACATCCGTGGGACAAGC TTCTGGGAAAACGTCAACGA 50.0 45.0 58.0 57.7 637-850 bp 0.985075 88.6
psaC-rps15_p2 GGGACAAGCTCGTACACATT TTCTGGGAAAACGTCAACGA 50.0 45.0 57.9 57.7 626-839 bp 0.985075 88.1
psaC-rps15_p3 ACAAGCTCGTACACATTGGG TTCTGGGAAAACGTCAACGA 50.0 45.0 58.2 57.7 623-836 bp 0.985075 87.4
psaC-rps15_p4 CAAGCTCGTACACATTGGGT TTCTGGGAAAACGTCAACGA 50.0 45.0 58.2 57.7 622-835 bp 0.985075 87.4
psaC-rps15_p5 ACATCCGTCCCAAGGTATCA TTCTGGGAAAACGTCAACGA 50.0 45.0 58.1 57.7 662-875 bp 0.970149 87.3