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trnL-UAG-rpl32

SSC · 120950-121382 · 433 bp

Back to Paphiopedilum

Pi 0.0491

MarkerSeek score 79.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 433
variable_sites: 179
pi: 0.0491
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TAGACACGCTGCTCTTAGGA	TGCCGCTAAAGAGCCTTTTT	50.0	45.0	57.9	58.4	446-701 bp	0.940299	85.5
trnL-UAG-rpl32_p2	TAGACACGCTGCTCTTAGGA	TTTACTGCCGCTAAAGAGCC	50.0	50.0	57.9	58.3	451-706 bp	0.880597	84.1
trnL-UAG-rpl32_p3	AAATTGGTAGACACGCTGCT	TTTACTGCCGCTAAAGAGCC	45.0	50.0	57.8	58.3	458-713 bp	0.880597	83.6
trnL-UAG-rpl32_p4	GGTAGACACGCTGCTCTTAG	TTTACTGCCGCTAAAGAGCC	55.0	50.0	57.8	58.3	453-708 bp	0.880597	83.5
trnL-UAG-rpl32_p5	GTAGACACGCTGCTCTTAGG	TTTACTGCCGCTAAAGAGCC	55.0	50.0	57.8	58.3	452-707 bp	0.880597	83.5