Hotspot

rps15-psaC

IRb · 117420-118069 · 650 bp

Back to Paphiopedilum

Pi 0.0201

MarkerSeek score 76.60

Primer available yes

Label rps15-psaC

Feature Metadata

label_name: rps15-psaC
feature_type: igs
region: IRb
length_bp: 650
variable_sites: 150
pi: 0.0201
unique_haplotype_count: 57
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-psaC_p1	TTCTGGGAAAACGTCAACGA	AATACATCCGTGGGACAAGC	45.0	50.0	57.7	58.0	637-850 bp	0.955224	87.4
rps15-psaC_p2	TTCTGGGAAAACGTCAACGA	GGGACAAGCTCGTACACATT	45.0	50.0	57.7	57.9	626-839 bp	0.955224	86.9
rps15-psaC_p3	TTCTGGGAAAACGTCAACGA	ACAAGCTCGTACACATTGGG	45.0	50.0	57.7	58.2	623-836 bp	0.955224	86.2
rps15-psaC_p4	TTCTGGGAAAACGTCAACGA	CAAGCTCGTACACATTGGGT	45.0	50.0	57.7	58.2	622-835 bp	0.955224	86.2
rps15-psaC_p5	TTCTGGGAAAACGTCAACGA	ACATCCGTCCCAAGGTATCA	45.0	50.0	57.7	58.1	662-875 bp	0.940299	85.6