Hotspot

trnP-UGG-psaJ

LSC · 66682-67442 · 761 bp

Back to Paphiopedilum

Pi 0.112
MarkerSeek score 89.20
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
761
variable_sites
540
pi
0.112
unique_haplotype_count
65
primer_available
yes
alignment_reliability
0.97
markerseek_score
89.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG TAGACCCGCTAAAGACCCAA 45.0 50.0 57.9 58.1 477-1337 bp 1.0 90.6
trnP-UGG-psaJ_p2 TGAACCCGCGACATTTTGTA TAGACCCGCTAAAGACCCAA 45.0 50.0 58.1 58.1 500-1360 bp 1.0 90.6
trnP-UGG-psaJ_p3 TTTGAACCCGCGACATTTTG TAGACCCGCTAAAGACCCAA 45.0 50.0 57.9 58.1 502-1362 bp 1.0 90.5
trnP-UGG-psaJ_p4 AGGGATGGCAGGATTTGAAC TAGACCCGCTAAAGACCCAA 50.0 50.0 57.9 58.1 515-1375 bp 1.0 90.3
trnP-UGG-psaJ_p5 CAAAACAAACGCGCTACCAA TAGACCCGCTAAAGACCCAA 45.0 50.0 58.2 58.1 478-1338 bp 1.0 90.2