Hotspot

psaA-ycf3

LSC · 43136-43775 · 640 bp

Back to Paphiopedilum

Pi 0.0244

MarkerSeek score 72.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 78
pi: 0.0244
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	642-861 bp	1.0	90.3
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	598-817 bp	1.0	89.6
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	597-816 bp	1.0	89.6
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	632-851 bp	1.0	88.9
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	601-820 bp	1.0	88.4