Hotspot

trnE-UUC-trnT-GGU

LSC · 31869-33372 · 1504 bp

Back to Paphiopedilum

Pi 0.121

MarkerSeek score 84.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1504
variable_sites: 986
pi: 0.121
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	584-2598 bp	0.970149	89.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	549-2563 bp	0.970149	88.6
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	562-2576 bp	0.970149	88.5
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	581-2595 bp	0.970149	88.2
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TTTTCCGCCGAAAAACGAAG	50.0	45.0	57.8	57.9	646-2660 bp	0.940299	87.4