Hotspot

trnC-GCA-petN

LSC · 28233-29016 · 784 bp

Back to Paphiopedilum

Pi 0.0256

MarkerSeek score 75.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 199
pi: 0.0256
unique_haplotype_count: 57
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	681-1205 bp	1.0	87.0
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	706-1230 bp	1.0	86.6
trnC-GCA-petN_p3	GACATGGCCAAGTGGTAAGG	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.5	58.2	727-1251 bp	1.0	86.5
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	CCCACTCCTTCCCCATACTA	50.0	55.0	58.5	57.5	738-1262 bp	1.0	84.3
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	CCCACTCCTTCCCCATACTA	50.0	55.0	57.5	57.5	763-1287 bp	1.0	83.9