Hotspot

trnR-UCU-atpA

LSC · 10371-10609 · 239 bp

Back to Paphiopedilum

Pi 0.0701

MarkerSeek score 75.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 89
pi: 0.0701
unique_haplotype_count: 51
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CACTGAAGAAGCTGAAACCCT	47.6	47.6	57.3	58.1	280-542 bp	1.0	69.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TCACTGAAGAAGCTGAAACCC	47.6	47.6	57.3	58.1	281-543 bp	1.0	69.3
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	ACTGAAGAAGCTGAAACCCT	47.6	45.0	57.3	56.7	279-541 bp	1.0	67.8
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	CACTGAAGAAGCTGAAACCC	47.6	50.0	57.3	56.6	280-542 bp	1.0	67.6
trnR-UCU-atpA_p5	GGGCGCCCATTGTCTAATG	CACTGAAGAAGCTGAAACCCT	57.9	47.6	59.0	58.1	332-594 bp	1.0	67.1