Hotspot

rps15-ycf1

SSC · 123062-123458 · 397 bp

Back to Papaver

Pi 0.0503

MarkerSeek score 71.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 397
variable_sites: 57
pi: 0.0503
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GATGAGTCGAAGAACGCCAT	ACATAAAGAACTGCGGCACT	50.0	45.0	58.1	57.8	999-1084 bp	0.733333	77.4
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	ACATAAAGAACTGCGGCACT	50.0	45.0	58.0	57.8	1124-1209 bp	0.733333	77.2
rps15-ycf1_p3	GCGCCTATATCCGCCATAAA	ACATAAAGAACTGCGGCACT	50.0	45.0	58.0	57.8	1292-1377 bp	0.733333	76.8
rps15-ycf1_p4	TTGTTTTGCTTCTTGCGCAA	ACATAAAGAACTGCGGCACT	40.0	45.0	58.0	57.8	652-662 bp	0.6	62.9
rps15-ycf1_p5	TTTGTTTTGCTTCTTGCGCA	ACATAAAGAACTGCGGCACT	40.0	45.0	58.0	57.8	653-663 bp	0.6	62.9