Hotspot

ccsA-ndhD

SSC · 115083-115324 · 242 bp

Back to Papaver

Pi 0.043

MarkerSeek score 66.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 242
variable_sites: 30
pi: 0.043
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GAGGCATGGGGGTCATATTG	TTCTCGATGGCTTCTCTTGC	55.0	50.0	58.1	58.0	793-836 bp	1.0	88.3
ccsA-ndhD_p2	AGGCACAAATTCCGCAATTG	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	686-728 bp	0.866667	82.5
ccsA-ndhD_p3	CAAGGCACAAATTCCGCAAT	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	688-730 bp	0.866667	82.5
ccsA-ndhD_p4	AGGCACAAATTCCGCAATTG	GCATATGGGTTGGTTCGGAT	45.0	50.0	57.9	58.0	1055-1097 bp	0.866667	80.6
ccsA-ndhD_p5	CAAGGCACAAATTCCGCAAT	GCATATGGGTTGGTTCGGAT	45.0	50.0	57.9	58.0	1057-1099 bp	0.866667	80.6