Hotspot

psaJ-rpl33

LSC · 66808-67197 · 390 bp

Back to Papaver

Pi 0.0322

MarkerSeek score 65.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 35
pi: 0.0322
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GTTTTGGAGACCCACGTTCT	ACGGTTTCGGATACAACTGG	50.0	50.0	58.0	57.9	1178-1206 bp	1.0	86.7
psaJ-rpl33_p2	GTTTTGGAGACCCACGTTCT	CGGTTTCGGATACAACTGGT	50.0	50.0	58.0	57.9	1177-1205 bp	1.0	86.7
psaJ-rpl33_p3	ATTCCTTTTGTGGGCCCAAT	CTCTCCCTCTGATTCGGTCT	45.0	55.0	58.0	57.9	1255-1268 bp	0.666667	67.6
psaJ-rpl33_p4	ATTCCTTTTGTGGGCCCAAT	CGGTTTCGGATACAACTGGT	45.0	50.0	58.0	57.9	1101-1114 bp	0.666667	67.6
psaJ-rpl33_p5	GTTTTGGAGACCCACGTTCT	CTCTCCCTCTGATTCGGTCT	50.0	55.0	58.0	57.9	1331-1344 bp	0.666667	67.5