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psbE-petL

LSC · 64302-65546 · 1245 bp

Back to Papaver

Pi 0.0273

MarkerSeek score 67.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 94
pi: 0.0273
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	TTGAAGCCGCCAGTAGAAAA	50.0	45.0	57.9	57.7	1390-1442 bp	1.0	84.7
psbE-petL_p2	TATCACCAAAAGCGCGTTCT	TTGAAGCCGCCAGTAGAAAA	45.0	45.0	58.2	57.7	1323-1375 bp	1.0	83.9
psbE-petL_p3	ACGAATAACCAACCCGCAAT	TTGAAGCCGCCAGTAGAAAA	45.0	45.0	58.2	57.7	1399-1451 bp	1.0	83.7
psbE-petL_p4	TGCTGACGAATAACCAACCC	TTGAAGCCGCCAGTAGAAAA	50.0	45.0	58.2	57.7	1404-1456 bp	1.0	83.6
psbE-petL_p5	CGAATAACCAACCCGCAATG	TTGAAGCCGCCAGTAGAAAA	50.0	45.0	57.8	57.7	1398-1450 bp	1.0	83.6