Hotspot

accD-psaI

LSC · 58735-59212 · 478 bp

Back to Papaver

Pi 0.0475

MarkerSeek score 74.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 58
pi: 0.0475
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTTCACGGTTTCTTTCCCTT	ATTGCAATTGCCGGGAAAAC	45.0	45.0	55.9	58.2	588-608 bp	1.0	71.1
accD-psaI_p2	CTTCACGGTTTCTTTCCCTT	CATTGCAATTGCCGGGAAAA	45.0	45.0	55.9	58.2	589-609 bp	1.0	71.1
accD-psaI_p3	CTTCACGGTTTCTTTCCCTT	GCAATTGCCGGGAAAACTAG	45.0	50.0	55.9	57.7	585-605 bp	1.0	70.4
accD-psaI_p4	CTTCACGGTTTCTTTCCCTT	TGCAATTGCCGGGAAAACTA	45.0	45.0	55.9	58.4	586-606 bp	1.0	69.6
accD-psaI_p5	CTTCACGGTTTCTTTCCCTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	55.9	57.9	596-610 bp	0.933333	68.6