Hotspot

ndhC-trnM-CAU

LSC · 50074-52301 · 2228 bp

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Pi 0.0269
MarkerSeek score 67.80
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
2228
variable_sites
169
pi
0.0269
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 TGCCCAGAAAATGTCATATTCG CCCGCCGTATGAAAGCAATA 40.9 50.0 57.1 58.4 2308-2378 bp 1.0 60.0
ndhC-trnM-CAU_p2 GCCCAGAAAATGTCATATTCGT CCCGCCGTATGAAAGCAATA 40.9 50.0 57.1 58.4 2307-2377 bp 1.0 60.0
ndhC-trnM-CAU_p3 AGAAATGCCCAGAAAATGTCA CCCGCCGTATGAAAGCAATA 38.1 50.0 56.0 58.4 2313-2383 bp 1.0 59.4
ndhC-trnM-CAU_p4 TGCCCAGAAAATGTCATATTCG CCGCCGTATGAAAGCAATAC 40.9 50.0 57.1 57.3 2307-2377 bp 1.0 58.0
ndhC-trnM-CAU_p5 GCCCAGAAAATGTCATATTCGT CCGCCGTATGAAAGCAATAC 40.9 50.0 57.1 57.3 2306-2376 bp 1.0 58.0