Hotspot

trnF-GAA-ndhJ

LSC · 47637-48386 · 750 bp

Back to Papaver

Pi 0.0322

MarkerSeek score 69.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 74
pi: 0.0322
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	775-887 bp	1.0	51.0
trnF-GAA-ndhJ_p2	AAGGGTTGGGATAGCTCAGT	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.0	53.8	818-930 bp	1.0	50.7
trnF-GAA-ndhJ_p3	AGGGTTGGGATAGCTCAGTT	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.0	53.8	817-929 bp	1.0	50.7
trnF-GAA-ndhJ_p4	GGGTTGGGATAGCTCAGTTG	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	816-928 bp	1.0	50.7
trnF-GAA-ndhJ_p5	GGTTGGGATAGCTCAGTTGG	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	815-927 bp	1.0	50.7