Hotspot

trnT-GGU-psbD

LSC · 31510-32809 · 1300 bp

Back to Papaver

Pi 0.0346

MarkerSeek score 70.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1300
variable_sites: 119
pi: 0.0346
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1462-1469 bp	1.0	86.1
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1476-1483 bp	1.0	85.9
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	50.0	50.0	57.9	57.7	1478-1485 bp	1.0	85.5
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1461-1468 bp	1.0	84.9
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1425-1432 bp	1.0	84.9