Hotspot

trnD-GUC-trnY-GUA

LSC · 30395-30763 · 369 bp

Back to Papaver

Pi 0.0313

MarkerSeek score 70.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 36
pi: 0.0313
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TGACGGATTCATCGGATCCT	TGGATTCTTCCTGGGTCGAT	50.0	50.0	58.3	58.1	546-563 bp	1.0	85.7
trnD-GUC-trnY-GUA_p2	TGAGTTGACGGATTCATCGG	ATGGATTCTTCCTGGGTCGA	50.0	50.0	57.7	58.1	552-569 bp	1.0	85.6
trnD-GUC-trnY-GUA_p3	TGAGTTGACGGATTCATCGG	TGGATTCTTCCTGGGTCGAT	50.0	50.0	57.7	58.1	551-568 bp	1.0	85.6
trnD-GUC-trnY-GUA_p4	TGACGGATTCATCGGATCCT	ATGGATTCTTCCTGGGTCGA	50.0	50.0	58.3	58.1	547-564 bp	1.0	85.6
trnD-GUC-trnY-GUA_p5	TTCATCGGATCCTAGGTCGG	TGGATTCTTCCTGGGTCGAT	55.0	50.0	58.4	58.1	539-556 bp	1.0	85.1