Hotspot

rpoB-trnC-GCA

LSC · 26868-27862 · 995 bp

Back to Papaver

Pi 0.0305

MarkerSeek score 68.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 89
pi: 0.0305
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CTCTGCTTCTTGCTTTTGCC	50.0	50.0	58.0	57.9	1659-1662 bp	0.666667	66.7
rpoB-trnC-GCA_p2	CCGGGGCTTTGCAATATTTG	CTCTGCTTCTTGCTTTTGCC	50.0	50.0	58.1	57.9	1539-1542 bp	0.666667	66.5
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TTCTCTCTTGGGTCCTAGGC	50.0	55.0	58.0	58.1	1749-1752 bp	0.666667	66.1
rpoB-trnC-GCA_p4	AATCTTCCTCCCCAATCGGA	CTCTGCTTCTTGCTTTTGCC	50.0	50.0	58.1	57.9	1620-1623 bp	0.666667	66.1
rpoB-trnC-GCA_p5	ATCTTCCTCCCCAATCGGAT	CTCTGCTTCTTGCTTTTGCC	50.0	50.0	57.9	57.9	1619-1622 bp	0.666667	65.9