Hotspot

psbI-trnS-GCU

LSC · 8069-8219 · 151 bp

Back to Papaver

Pi 0.0512

MarkerSeek score 68.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 20
pi: 0.0512
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAGGA	TTCGAATCCCTCTCTTTCCG	55.0	50.0	58.9	57.0	191-217 bp	1.0	77.1
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAGGA	GGGTTCGAATCCCTCTCTTT	55.0	50.0	58.9	56.9	194-220 bp	1.0	76.1
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAGGA	GGGTTCGAATCCCTCTCTTTC	55.0	52.4	58.9	58.1	194-220 bp	1.0	76.1
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAGGA	GGTTCGAATCCCTCTCTTTCC	55.0	52.4	58.9	58.1	193-219 bp	1.0	76.1
psbI-trnS-GCU_p5	GTAATCCTGGACGTGAGGAA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	56.7	57.0	190-216 bp	1.0	73.6