Hotspot

ccsA-ndhD

SSC · 106778-106985 · 208 bp

Back to Panicum

Pi 0.0332

MarkerSeek score 70.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 208
variable_sites: 18
pi: 0.0332
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAACTCTTTTGTGCGGATC	ACAGCTTGTGATAGAGTGCG	50.0	50.0	58.0	58.0	1171-1195 bp	1.0	85.0
ccsA-ndhD_p2	GCAACTCTTTTGTGCGGATC	CAGCTTGTGATAGAGTGCGT	50.0	50.0	58.0	58.0	1170-1194 bp	1.0	85.0
ccsA-ndhD_p3	GCAACTCTTTTGTGCGGATC	ATTCTGGACCACGGGAACTA	50.0	50.0	58.0	58.1	870-879 bp	0.714286	71.3
ccsA-ndhD_p4	GCAACTCTTTTGTGCGGATC	TCTGGACCACGGGAACTATT	50.0	50.0	58.0	58.1	868-877 bp	0.714286	71.3
ccsA-ndhD_p5	GCAACTCTTTTGTGCGGATC	TTCTGGACCACGGGAACTAT	50.0	50.0	58.0	58.1	869-878 bp	0.714286	71.3