Hotspot

rpl32-trnL-UAG

SSC · 105136-105651 · 516 bp

Back to Panicum

Pi 0.0386

MarkerSeek score 77.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 516
variable_sites: 59
pi: 0.0386
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAACAAACAAATAATAAGGTTTTGGA	TCCTAAGAGCAGCGTGTCTA	26.9	50.0	55.7	57.9	570-590 bp	1.0	50.2
rpl32-trnL-UAG_p2	CAACAAACAAATAATAAGGTTTTGGA	AGCAGCGTGTCTACCAATTT	26.9	45.0	55.7	57.8	563-583 bp	1.0	50.2
rpl32-trnL-UAG_p3	CAACAAACAAATAATAAGGTTTTGGA	CCTAAGAGCAGCGTGTCTAC	26.9	55.0	55.7	57.8	569-589 bp	1.0	50.2
rpl32-trnL-UAG_p4	CAACAAACAAATAATAAGGTTTTGGA	CTAAGAGCAGCGTGTCTACC	26.9	55.0	55.7	57.8	568-588 bp	1.0	50.2
rpl32-trnL-UAG_p5	CAACAAACAAATAATAAGGTTTTGGA	GCTTGAGCACTGCTTCCTAA	26.9	50.0	55.7	58.2	584-604 bp	1.0	50.0