Hotspot

ndhF-rpl32

SSC · 104090-104943 · 854 bp

Back to Panicum

Pi 0.0254

MarkerSeek score 70.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 854
variable_sites: 58
pi: 0.0254
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGACGTAAGGGGGTCGATC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	1809-1887 bp	1.0	88.2
ndhF-rpl32_p2	TAGACGTAAGGGGGTCGATC	AATTTCACCATGGCGGCTAA	55.0	45.0	58.0	58.2	1787-1865 bp	1.0	88.0
ndhF-rpl32_p3	TAGACGTAAGGGGGTCGATC	TCGTTGCCGCTAGAAAATGA	55.0	45.0	58.0	57.9	1192-1271 bp	1.0	87.2
ndhF-rpl32_p4	ACACATTCCGACAAGTTCCC	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.1	57.9	1380-1459 bp	1.0	86.1
ndhF-rpl32_p5	CACATTCCGACAAGTTCCCA	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.1	57.9	1379-1458 bp	1.0	86.1