Hotspot

petD-rpoA

LSC · 75436-75650 · 215 bp

Back to Panicum

Pi 0.0313

MarkerSeek score 71.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 21
pi: 0.0313
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	AAATCCTTAACTTTAGGTCTTTTT	AGCATCTCCCAATTGATTTACCT	25.0	39.1	52.8	57.9	286-294 bp	1.0	48.6
petD-rpoA_p2	AAATCCTTAACTTTAGGTCTTTTT	AGCATCTCCCAATTGATTTACC	25.0	40.9	52.8	56.5	286-294 bp	1.0	48.6
petD-rpoA_p3	AAATCCTTAACTTTAGGTCTTTTT	GCATCTCCCAATTGATTTACCT	25.0	40.9	52.8	56.5	285-293 bp	1.0	48.6
petD-rpoA_p4	AAATCCTTAACTTTAGGTCTTTTT	AAGCATCTCCCAATTGATTTACC	25.0	39.1	52.8	57.1	287-295 bp	1.0	48.6
petD-rpoA_p5	AAATCCTTAACTTTAGGTCTTTTT	GCATCTCCCAATTGATTTACC	25.0	42.9	52.8	54.9	285-293 bp	1.0	48.6