Hotspot

trnF-GAA-ndhJ

LSC · 49234-49840 · 607 bp

Back to Panicum

Pi 0.0352

MarkerSeek score 81.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 607
variable_sites: 52
pi: 0.0352
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	662-717 bp	1.0	88.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	652-707 bp	1.0	88.1
trnF-GAA-ndhJ_p3	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	715-770 bp	1.0	87.9
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	705-760 bp	1.0	87.4
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	713-768 bp	1.0	86.9