Hotspot

trnR-UCU-trnfM-CAU

LSC · 37534-37699 · 166 bp

Back to Panicum

Pi 0.0663

MarkerSeek score 74.90

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 27
pi: 0.0663
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	ACAAAGCTCATAACCTTGGGG	47.6	47.6	56.9	58.2	236-242 bp	1.0	64.1
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	ACAAAGCTCATAACCTTGGGG	45.5	47.6	57.9	58.2	242-248 bp	1.0	63.4
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	TTGGGGTTACGGGTTCGAT	47.6	52.6	56.9	58.6	221-227 bp	1.0	60.8
trnR-UCU-trnfM-CAU_p4	AGAAGACCTCTGTCCTATCCA	CAAAGCTCATAACCTTGGGG	47.6	50.0	56.9	56.4	235-241 bp	1.0	60.6
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCAT	ACAAAGCTCATAACCTTGGGG	45.5	47.6	57.4	58.2	236-242 bp	1.0	60.1