Hotspot

trnD-GUC-psbM

LSC · 16886-17928 · 1043 bp

Back to Panicum

Pi 0.0267

MarkerSeek score 73.90

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 72
pi: 0.0267
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	844-1242 bp	1.0	74.8
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	846-1244 bp	1.0	73.0
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	845-1243 bp	1.0	73.0
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.5	57.4	844-1242 bp	1.0	72.8
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	TTTGGCTGGCTGTTTTTACA	50.0	40.0	58.5	56.3	840-1238 bp	1.0	72.0