Hotspot

trnG-UCC-trnM-CAU

LSC · 13715-15678 · 1964 bp

Back to Panicum

Pi 0.0283
MarkerSeek score 74.10
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1964
variable_sites
152
pi
0.0283
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1382-2048 bp 1.0 51.2
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1378-2044 bp 1.0 50.5
trnG-UCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1379-2045 bp 1.0 50.5
trnG-UCC-trnM-CAU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1385-2051 bp 1.0 50.4
trnG-UCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1384-2050 bp 1.0 50.4