Hotspot

psbK-psbI

LSC · 7179-7563 · 385 bp

Back to Panicum

Pi 0.0221

MarkerSeek score 70.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 22
pi: 0.0221
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	GGATTACGTCCTGGGTCATT	50.0	50.0	58.0	57.0	499-513 bp	1.0	77.8
psbK-psbI_p2	CGTGGATGTTATGCCTGTCA	GGATTACGTCCTGGGTCATT	50.0	50.0	58.0	57.0	554-568 bp	1.0	77.4
psbK-psbI_p3	AGCCGCTGTAAGTTTTCGAT	GGATTACGTCCTGGGTCATT	45.0	50.0	57.9	57.0	497-511 bp	1.0	77.4
psbK-psbI_p4	AAGCCGCTGTAAGTTTTCGA	GGATTACGTCCTGGGTCATT	45.0	50.0	58.1	57.0	498-512 bp	1.0	77.2
psbK-psbI_p5	ACCCGAAGCTTATGCCATTT	GGATTACGTCCTGGGTCATT	45.0	50.0	57.9	57.0	584-598 bp	1.0	76.1