Hotspot

clpP1

LSC · 71483-73537 · 2055 bp

Back to Pandanus

Pi 0.0116

MarkerSeek score 53.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2055
variable_sites: 32
pi: 0.0116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGGTTTGAAATGGAATTGTAGA	TCTATGAACCTTCCAATTTATGT	31.8	30.4	53.9	53.4	2181-2302 bp	1.0	42.9
clpP1_p2	TGGTTTGAAATGGAATTGTAGAT	TCTATGAACCTTCCAATTTATGT	30.4	30.4	54.5	53.4	2181-2302 bp	1.0	42.9
clpP1_p3	TGGTTTGAAATGGAATTGTAG	TCTATGAACCTTCCAATTTATGT	33.3	30.4	52.3	53.4	2181-2302 bp	1.0	42.9
clpP1_p4	GGTTTGAAATGGAATTGTAGA	TCTATGAACCTTCCAATTTATGT	33.3	30.4	52.0	53.4	2180-2301 bp	1.0	42.9
clpP1_p5	TGGTTTGAAATGGAATTGTAGA	TCTATGAACCTTCCAATTTATGTA	31.8	29.2	53.9	53.5	2181-2302 bp	1.0	42.9