Hotspot

trnN-GUU-trnR-ACG

IRa · 132470-133060 · 591 bp

Back to Pandanus

Pi 0.00457

MarkerSeek score 62.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 591
variable_sites: 4
pi: 0.00457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	728-732 bp	1.0	79.6
trnN-GUU-trnR-ACG_p2	CGGTTGGCTGTTAACTGACT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	698-702 bp	1.0	78.7
trnN-GUU-trnR-ACG_p3	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	692-696 bp	1.0	78.5
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	679-683 bp	1.0	78.4
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	680-684 bp	1.0	78.0