Hotspot

trnL-UAG-rpl32

SSC · 126767-127550 · 784 bp

Back to Pandanus

Pi 0.0494

MarkerSeek score 90.50

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 784
variable_sites: 58
pi: 0.0494
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	TAAATCGATTTCCACGGGGC	50.0	50.0	58.0	58.3	871-898 bp	1.0	82.8
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	TAAATCGATTTCCACGGGGC	50.0	50.0	58.0	58.3	872-899 bp	1.0	82.7
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	ATTTCCACGGGGCATTCAAA	50.0	45.0	58.0	58.4	864-891 bp	1.0	82.5
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	ATTTCCACGGGGCATTCAAA	50.0	45.0	58.0	58.4	865-892 bp	1.0	82.5
trnL-UAG-rpl32_p5	TTTAGAAGATGCCGCCACTC	TAAATCGATTTCCACGGGGC	50.0	50.0	58.0	58.3	931-958 bp	1.0	82.3