Hotspot

psbB-psbT

LSC · 75504-75678 · 175 bp

Back to Pandanus

Pi 0.0229

MarkerSeek score 56.20

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 5
pi: 0.0229
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAACTACAAAGAGACAGGT	CTTTAGGCGGGTCTCGAAAA	42.9	50.0	56.2	57.9	288-294 bp	1.0	59.0
psbB-psbT_p2	GATCCAACTACAAAGAGACAGGT	CTTTAGGCGGGTCTCGAAAA	43.5	50.0	57.8	57.9	290-296 bp	1.0	56.0
psbB-psbT_p3	TCCAACTACAAAGAGACAGGT	TTTAGGCGGGTCTCGAAAAA	42.9	45.0	56.2	57.5	287-293 bp	1.0	55.3
psbB-psbT_p4	ATCCAACTACAAAGAGACAGGT	CTTTAGGCGGGTCTCGAAAA	40.9	50.0	56.7	57.9	289-295 bp	1.0	55.2
psbB-psbT_p5	TCCAACTACAAAGAGACAGGT	TTAGTCGGAACTTTAGGCGG	42.9	50.0	56.2	57.3	298-304 bp	1.0	54.6