Hotspot

rps18-rpl20

LSC · 69848-70101 · 254 bp

Back to Pandanus

Pi 0.00787

MarkerSeek score 62.30

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 3
pi: 0.00787
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	CCGAGTCGATCCCTAGAACT	ACAATCTGTACAAAAAGCAATTGC	55.0	33.3	58.0	57.5	385-420 bp	1.0	48.1
rps18-rpl20_p2	ATCCCTAGAACTACTGGCCC	ACAATCTGTACAAAAAGCAATTGC	55.0	33.3	57.9	57.5	377-412 bp	1.0	47.5
rps18-rpl20_p3	AATTTGAGAGAGCCGAGTCG	ACAATCTGTACAAAAAGCAATTGC	50.0	33.3	57.7	57.5	397-432 bp	1.0	46.0
rps18-rpl20_p4	AACTACTGGCCCTAGAACCA	ACAATCTGTACAAAAAGCAATTGC	50.0	33.3	57.7	57.5	369-404 bp	1.0	45.7
rps18-rpl20_p5	TTTGAGAGAGCCGAGTCGAT	ACAATCTGTACAAAAAGCAATTGC	50.0	33.3	58.5	57.5	395-430 bp	1.0	44.6