Hotspot

pafII-cemA

LSC · 61609-62421 · 813 bp

Back to Pandanus

Pi 0.0121

MarkerSeek score 65.80

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 13
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	GAGCCATTCCATTGACTCGT	TAACCCAGGGTTCCAGACTT	50.0	50.0	58.0	57.9	977-1146 bp	1.0	83.7
pafII-cemA_p2	ACAAAAAGCTGCCGAATTGG	TAACCCAGGGTTCCAGACTT	45.0	50.0	57.8	57.9	925-1094 bp	1.0	82.8
pafII-cemA_p3	TATTTCTTGCGCGTACCGAT	TAACCCAGGGTTCCAGACTT	45.0	50.0	57.8	57.9	903-1072 bp	1.0	82.3
pafII-cemA_p4	CTATTTCTTGCGCGTACCGA	TAACCCAGGGTTCCAGACTT	50.0	50.0	58.4	57.9	904-1073 bp	1.0	80.5
pafII-cemA_p5	GCGCGTACCGATTGAAGTAT	TAACCCAGGGTTCCAGACTT	50.0	50.0	58.5	57.9	895-1064 bp	1.0	79.9