Hotspot

trnT-UGU-trnL-UAA

LSC · 46756-47558 · 803 bp

Back to Pandanus

Pi 0.0159
MarkerSeek score 62.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
803
variable_sites
19
pi
0.0159
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AGAGAAAAAGCCGGCTATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.1 58.0 904-1440 bp 1.0 81.1
trnT-UGU-trnL-UAA_p2 AGAGAAAAAGCCGGCTATCG TAGCGTCTACCAATTTCGCC 50.0 50.0 58.1 58.1 912-1448 bp 1.0 80.6
trnT-UGU-trnL-UAA_p3 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 884-1420 bp 1.0 80.4
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC TAGCGTCTACCAATTTCGCC 55.0 50.0 58.2 58.1 892-1428 bp 1.0 79.8
trnT-UGU-trnL-UAA_p5 AGAGAAAAAGCCGGCTATCG ATCCAATCAAGTCCGTAGCG 50.0 50.0 58.1 57.8 927-1463 bp 1.0 79.3