Hotspot

psaA-pafI

LSC · 42178-42802 · 625 bp

Back to Pandanus

Pi 0.00537

MarkerSeek score 62.80

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 625
variable_sites: 5
pi: 0.00537
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	CCCATTCCTCGAAAGACGTT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	808-825 bp	1.0	79.8
psaA-pafI_p2	CCCATTCCTCGAAAGACGTT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	810-827 bp	1.0	79.0
psaA-pafI_p3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	737-754 bp	1.0	78.9
psaA-pafI_p4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	741-758 bp	1.0	78.7
psaA-pafI_p5	CCCATTCCTCGAAAGACGTT	AGCTGCCGAGTATTGGAAAC	50.0	50.0	57.9	58.3	804-821 bp	1.0	78.0