Hotspot

petN-psbM

LSC · 28430-29644 · 1215 bp

Back to Pandanus

Pi 0.00786

MarkerSeek score 59.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1215
variable_sites: 13
pi: 0.00786
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1309-1356 bp	1.0	71.3
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1308-1355 bp	1.0	71.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1367-1414 bp	1.0	70.9
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	1307-1354 bp	1.0	70.9
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.2	57.6	1304-1351 bp	1.0	70.6