Hotspot

trnK-UUU-rps16

LSC · 4140-4702 · 563 bp

Back to Pandanus

Pi 0.00838
MarkerSeek score 62.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
563
variable_sites
7
pi
0.00838
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 672-677 bp 1.0 71.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 671-676 bp 1.0 71.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GAAAAGGGCGCTCAACCTAC 50.0 55.0 58.2 59.2 673-678 bp 1.0 70.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GAAAAGGGCGCTCAACCTAC 50.0 55.0 58.2 59.2 672-677 bp 1.0 70.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GAAAAGGGCGCTCAACCTA 50.0 52.6 58.2 57.8 672-677 bp 1.0 70.3