Hotspot

rpl16

LSC · 83791-85172 · 1382 bp

Back to Pancratium

Pi 0.00728

MarkerSeek score 50.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1382
variable_sites: 10
pi: 0.00728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGGGCACTGCTTCTTTGA	GCCATTCTGTCCAGTGGTAG	45.0	55.0	58.0	58.0	1789-1797 bp	1.0	80.8
rpl16_p2	TGGGCACTGCTTCTTTGATT	GCCATTCTGTCCAGTGGTAG	45.0	55.0	58.0	58.0	1787-1795 bp	1.0	80.8
rpl16_p3	TTGGGCACTGCTTCTTTGAT	GCCATTCTGTCCAGTGGTAG	45.0	55.0	58.0	58.0	1788-1796 bp	1.0	80.8
rpl16_p4	CGCACCAAAAACTCGAGTTC	GCCATTCTGTCCAGTGGTAG	50.0	55.0	58.0	58.0	1945-1953 bp	1.0	80.6
rpl16_p5	GTCTCTGCCCATGACGAATT	GCCATTCTGTCCAGTGGTAG	50.0	55.0	58.0	58.0	2219-2228 bp	1.0	80.5