Hotspot

rps8-rpl14

LSC · 83093-83289 · 197 bp

Back to Pancratium

Pi 0.0153

MarkerSeek score 61.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 3
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GTGGATGCTATTCGAACCGT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.1	58.0	368-369 bp	1.0	80.2
rps8-rpl14_p2	GTGGATGCTATTCGAACCGT	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	58.1	58.1	366-367 bp	1.0	79.4
rps8-rpl14_p3	AGTGGATGCTATTCGAACCG	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.8	58.0	369-370 bp	1.0	78.9
rps8-rpl14_p4	AGTGGATGCTATTCGAACCG	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	57.8	58.1	367-368 bp	1.0	78.1
rps8-rpl14_p5	GTGGATGCTATTCGAACCGT	AACTCGAGTTTTTGGTGCGA	50.0	45.0	58.1	58.3	367-368 bp	1.0	77.6