Hotspot

petA-psbJ

LSC · 65501-66683 · 1183 bp

Back to Pancratium

Pi 0.00343

MarkerSeek score 62.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 4
pi: 0.00343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1290-1308 bp	1.0	79.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1291-1309 bp	1.0	79.2
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.7	58.0	1297-1315 bp	1.0	75.6
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.7	58.1	1298-1316 bp	1.0	75.0
petA-psbJ_p5	GATCCATTACGCGTCCAAGG	CCGGATTGGGTTCATCTCTG	55.0	55.0	58.8	58.0	1308-1326 bp	1.0	74.8