Hotspot

ndhC-trnV-UAC

LSC · 52290-53877 · 1588 bp

Back to Pancratium

Pi 0.00567

MarkerSeek score 61.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1588
variable_sites: 9
pi: 0.00567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1719 bp	1.0	77.7
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1801 bp	1.0	75.5
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1732 bp	1.0	75.1
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1718 bp	1.0	75.0
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.4	57.8	1736 bp	1.0	74.0